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Oxford Instruments
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ANSYS inc
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Accelrys
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BARCO Inc
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Geomagic Inc
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Journal: bioRxiv
Article Title: Cross Sectional and Longitudinal Imaging Reveals Spatiotemporal Divergence in Morphogenesis and Cell Lineage Specification between in-vivo and in-vitro Mouse Embryo during Pre- and Peri-implantation
doi: 10.64898/2025.12.15.691388
Figure Lengend Snippet: (Aa,Ab) Changes in protrusion morphology of migrating PrE cells over time via green channel and bright field (BF) in 3D and 2D views. White arrows indicate migrating pioneer cells, with protrusions denoted by green arrows in the enlarged 3D viewer. Blue and red spots highlighted by white circles, added via IMARIS, confirm cell positions. Scale bar: 20μm. (B) Total embryo cell counts at the observation of the first migration cells featuring protrusions. (C) The timing of migration initiation in embryonic developmental day. In both (B,C), Mann Whitney test was employed. Error bars: SD. “Ff” denotes freshly flushed, mirroring the in-vivo group, and “CM” reflects the in-vitro group. Statistical significance: p = 0.05, * p < 0.05 and *** p < 0.001. The embryo sample size: Seven (three litters) for the in-vivo group and 10 (three videos across four litters) for the in-vitro group.
Article Snippet: An
Techniques: Migration, MANN-WHITNEY, In Vivo, In Vitro
Journal: bioRxiv
Article Title: Cross Sectional and Longitudinal Imaging Reveals Spatiotemporal Divergence in Morphogenesis and Cell Lineage Specification between in-vivo and in-vitro Mouse Embryo during Pre- and Peri-implantation
doi: 10.64898/2025.12.15.691388
Figure Lengend Snippet: (A) Sample sizes of in-vivo (blue) and in-vitro (green) embryos and Sox2- and Gata4-ICM cells. (B) Distance measurement between cells and the XY plane. The XY plane is set at the level of the P-TE apex and is oriented horizontally, perpendicular to the embryonic-abembryonic axis. The Z-axis runs parallel to the embryonic-abembryonic direction. The origin of the 3D coordinate system is at the P-TE apex, and “depth” refers to the distance of the cells from the XY plane along the Z-axis. (Ca,Cb) Depth of Gata4- (red) and Sox2-positive (grey) cells in vivo at E3.75 and E4.5, respectively. (Dc-e) As (C) but for E3.75, E4.375 and E4.5 in vitro .
Article Snippet: An
Techniques: In Vivo, In Vitro